Reporter

Part:BBa_K895007:Design

Designed by: Frank Sargent   Group: iGEM12_Dundee   (2012-09-23)

Tetrathionate-sensing two-component system linked to GFP production.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2550
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1179
    Illegal AgeI site found at 311
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 28
    Illegal BsaI.rc site found at 3199


Design Notes

The GFP ATG is positioned immediately downstream of an engineered BamHI site. This places the start codon at exactly the correct distance from the ttrB RBS.


Source

This a combination of BBa_K895003 and BBa_K895006 cloned together by PCR.

References

Hensel,M., Hinsley,A.P., Nikolaus,T., Sawers,G. and Berks,B.C. (1999) The genetic basis of tetrathionate respiration in Salmonella typhimurium. Mol. Microbiol. 32:275-287.